Non-targeted and Suspect Screening of DSS-TOX Chemicals in Human Serum Samples
Oral Presentation
Prepared by M. Russell, M. Strynar, S. Newton, R. McMahen, A. Lindstrom
ASPPH Environmental Health Fellowship Program, 109 TW Alexander Drive, Research Triangle Park, NC, 27711, United States
Contact Information: marie.clare.russell@gmail.com; 919-541-1058
ABSTRACT
Thousands of chemicals are found in environmental media (water, house dust etc.) that can lead to human exposures. We have developed and presented time-of-flight mass spectrometry (TOFMS) techniques for non-targeted and suspect-screening of chemicals in environmental media. However, biomonitoring for compounds found in the environment is a critical link in the “exposome”. The EPA DSS-TOX database contains bioactivity screening and toxicity information for thousands of chemicals. This information was utilized to identify high-risk chemicals that are present in both environmental and biomonitoring media. This effort will present data from a pilot scale application of suspect screening and non-targeted screening in human serum samples. De-identified serum samples from the Research Triangle Park (RTP), North Carolina, area were extracted and analyzed on an Agilent 6200 series TOFMS, operated in positive and negative mode. Results presented will include prioritization based on bioactivity, detection frequency, and abundance of chemicals found in the serum samples.
Oral Presentation
Prepared by M. Russell, M. Strynar, S. Newton, R. McMahen, A. Lindstrom
ASPPH Environmental Health Fellowship Program, 109 TW Alexander Drive, Research Triangle Park, NC, 27711, United States
Contact Information: marie.clare.russell@gmail.com; 919-541-1058
ABSTRACT
Thousands of chemicals are found in environmental media (water, house dust etc.) that can lead to human exposures. We have developed and presented time-of-flight mass spectrometry (TOFMS) techniques for non-targeted and suspect-screening of chemicals in environmental media. However, biomonitoring for compounds found in the environment is a critical link in the “exposome”. The EPA DSS-TOX database contains bioactivity screening and toxicity information for thousands of chemicals. This information was utilized to identify high-risk chemicals that are present in both environmental and biomonitoring media. This effort will present data from a pilot scale application of suspect screening and non-targeted screening in human serum samples. De-identified serum samples from the Research Triangle Park (RTP), North Carolina, area were extracted and analyzed on an Agilent 6200 series TOFMS, operated in positive and negative mode. Results presented will include prioritization based on bioactivity, detection frequency, and abundance of chemicals found in the serum samples.
