Keeping up with Changing PCR Methods

Oral Presentation

Prepared by J. Griffith
Southern California Coastal Water Research Project - Department of Microbiology, 3535 Harbor Blvd, Suite 110, Costa Mesa, CA, 92626, United States

Contact Information: [email protected]; 714-755-3228


qPCR was only recently approved by EPA for recreational water quality monitoring and is only beginning to be adopted by the states. Yet, newer PCR technology, known as digital droplet PCR (ddPCR), that boasts greater accuracy and is less susceptible to interference from common contaminants found in environmental water samples is poised to replace it. This presentation will focus on the changing landscape of quantitative PCR technology and how advances in chemistry and instrumentation are likely to revolutionize detection and quantification of microbes in water.