Optimization of Gas Chromatography-Triple Quadrupole Mass Spectrometry for the Determination of Underderivatized Triclosan and Related Compounds

Poster Presentation

Prepared by E. Mateus, C. Magro, D. Mendes, A. Ribeiro
CENSE/FCT-NOVA, FCT-NOVA, CAMPUS CAPARICA, DCEA, Caparica, not aplicable, 2829 516, Portugal

Contact Information: [email protected]; 00351212948300


Triclosan (TCS), an antimicrobial agent, has been ubiquitously found in wastewater and sewage sludge. TCS may suffer transformation or degradation during the wastewater treatment. Some of the resulted products such as 2,4-dichlorophenol and methyl-triclosan are presumed toxic/persistent compounds.
Liquid chromatography and mass spectrometry have become one of the most widely used techniques for identify and quantify PPCPs in water samples, due to its high sensitivity, specificity and absence of limitations with the non-volatility and thermal stability of compounds. However, gas chromatography (GC) may still offer better sensitivity and lower detection limits for complex sample investigations.
This work aims to study and develop an effective and non-use of derivatization method using GC with triple quadrupole mass spectrometry (GC/MS/MS) for the determination of TCS and related compounds in effluent from urban wastewater treatment plants and other aqueous matrices. The effect of the different GC/MS/MS parameters such as injector temperature, transfer line, source, collision gas pressure and energy, injection modes: split, splitless, pulse and liners on the qualitative and quantitative signal outputs were studied. Full scan, SIM and MRM modes were also investigated. The range of standard concentration for all tests were: 1ppm to 1ppt.
After method optimization, the analytical procedure that is appropriate for the quantitative and qualitative analysis of TCS was established and evaluated.
Acknowledgements: This work has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sk?odowska-Curie grant agreement No 778045. 74/2016 and National project “Development of Nanostrutures for Detection of Triclosan Traces on Aquatic Environments” (PTDC/FIS-NAN/0909/2014). CENSE-Center for Environmental and Sustainability Research which is financed by national funds from FCT/MEC (UID/AMB/04085/2013). C. Magro acknowledges Fundação para a Ciência e a Tecnologia for her PhD fellowship (SFRH/BD/1146).