Use of Innovative Enzymatic Methods for the Determination of Pseudomonas aeruginosa in Spas, Pools and Hospital Waters

Oral Presentation

Prepared by G. Dichter
IDEXX, One IDEXX Drive, Westbrook, ME, 04092, United States

Contact Information:; 207-556-4687


Pseudomonas aeruginosa is an opportunistic pathogen and is resistant to chlorination. It is a common inhabitant of water and can persist under low nutrient conditions. It can grow in a temperature range of 5-42°C and thrives in warm and humid conditions such as pools and spas. It can accumulate on surfaces and in plumbing and can result in a biofilm buildup.

Presently there are membrane filtration methods requiring 48-72 hours to obtain results. Confirmation may be required. The methods are time consuming requiring preparation and QC of the reagents. It is important to obtain results as quickly as possible to implement corrective action.

An innovative MPN enzymatic method, “Pseudalert” is a rapid, easy method for detection of Pseudomonas aeruginosa yielding results within 24 hours and is specific requiring no confirmation.

An introduction to Pseudomonas aeruginosa microbiology, as well a comparison of present multiple tube fermentation and membrane filtration methods comparing it to Pseudalert will be reviewed as well as to the importance for testing for Pseudomonas aeruginosa.

Studies will be presented comparing methods for pools, spas and hospital waters to showing that the rapid new enzymatic method is equivalent to membrane filtration methods with a quicker time to results taking correction action much sooner.