Rapid Determination of Ethynylestradiol (17EE2) in Wastewater Using EQuan On-line SPE and Q Exactive Focus Orbitrap LC-MS/MS

Poster-Vendor
Poster Presentation

Presented by R. Jack
Prepared by N. Llewellyn1, O. Scheibner2, J. Beck3, R. Jack4, G. Woffendin1
1 - Thermo Fisher Scientific, Stafford House, 1 Boundary Park,, Hemel Hempstead, Hemel Hempstead, HP2 7GE, United Kingdom
2 - Thermo Fisher Scientific, Im Steingrund 4, Dreieich, Dreieich, 63303, Germany
3 - Thermo Fisher Scientific, 355 River Oaks Pkwy, San Jose, CA, 95134, United States
4 - Thermo Fisher Scientific, 490 Lakeside Drive, Sunnyvale, CA, 94085, United States


Contact Information: neville.llewellyn@thermofisher.com; +44 1442 233555


ABSTRACT

The occurrence and effects of endocrine disrupting compounds (EDCs) in aquatic environments is a significant concern. Of the many EDCs, 17EE2 is recognised as possessing the greatest estogenic potency and risk to freshwater ecosystems and drinking water resources.Due to its environmental significance, 17EE2 was incorporated into the EU Water Framework Directive, with a stipulated Limit of Detection of 35 pg/L, which presents a significant analytical challenge.Current methods involve large-volume SPE; normal phase SPE clean up and size exclusion fractionation, which take considerable time, expense, and sampling logistics.The aim of this work is to assess the possibility of using on-line solid phase extraction and a Q Exactive Focus Orbitrap, for the determination of 17EE2 at the WFD LoD.