Analysis of Perfluoroalkyl and Polyfluoroalkyl Substances in Surface Water and Wastewater by EPA Method 8327

Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by J. Wu1, M. Costanzo2, J. Weisenseel2, T. Ordinario1, F. Qin1
1 - PerkinElmer Health Sciences Canada, Inc., 501 Rowntree Dairy Road, Unit 6, Woodbridge, ON, L4L 8H1, Canada
2 - PerkinElmer Inc., 710 Bridgeport Avenue, Shelton, CT, 06484, United States

Contact Information:; 647-539-4660


A fast and sensitive method for the analysis of PFAS and their surrogates based on EPA Method 8327 was developed and validated using QSight UHPLC-MS/MS. The sample preparation method involved a simple sample dilution and filtration. Excellent linearity was achieved for all analytes ranging from 5 ng/L to 100 ng/L by this method with LLOQ values lower than those listed in EPA Method 8327. The method was applied to the analysis of PFAS in reagent water, surface water and waste water samples. The reagent water samples were fortified with PFAS and their surrogates at three concentration levels (40, 80 and 160 ng/L, respectively) and the recoveries ranged from 72 – 109%. The wastewater, upstream surface water, and downstream surface water were fortified with PFAS surrogates at 160ng/L and the recoveries were between 82-104% with relative standard deviations (RSDs) <15%. The results demonstrates that the method has high accuracy and sensitivity and can be applied to monitor PFAS in non-potable waters.