Considerations for Improved EPA Method 533 Accuracy and Precision for the Analysis of PFAS in a Production Laboratory Environment

Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by S. Lodge1, A. Pierri2, R. Jack1
1 - Phenomenex, Inc., 411 Madrid Ave., Torrance, CA, 90501, United States
2 - Weck Laboratories, 14859 Clark Ave., Industry, CA, 91745, United States

Contact Information:; (310) 212-0555


EPA method 533 is designed to measure 18 PFAS analytes in drinking water and will be the central analytical method in the upcoming UCMR 5 campaign. Method 533 defines the basic method criteria that must be followed (WAX-SPE sample preparation, LC-MS/MS analytical platform, etc.), but allows flexibility in applying certain operational parameters, such as LC gradient composition, LC column selection, etc. It is relatively easy to meet Method 533 performance criteria while employing any combination of these optional parameters variables (for example, virtually any C18 HPLC column will produce acceptable results). However, in a commercial laboratory production environment, these variable parameters must be optimized to allow high sample throughput while still meeting method- mandated accuracy and precision requirements. This poster will describe such an optimized Method 533 production scheme and will provide accuracy and precision results developed by a commercial laboratory for the analysis of the matrix spikes, matrix spike duplicates, blank spikes and blank spike duplicates associated with the analysis of drinking water samples.