Development of Ultrashort-Chain (C2, C3) PFAS HPLC Methodology Utilizing Phenyl Column Chemistries

Polyfluoroalkyl Substances (PFAS) in the Environment
Oral Presentation

Prepared by J. Preston, Z. Jalali, R. Jack, D. Kennedy, S. Lodge
Phenomenex Inc, 411 Madrid, Torrance, CA, 90501, United States

Contact Information:; 310 212 0555


“PFAS analysis” is a simple phrase that covers a huge range of topics. Water samples are a large segment of these topics, but there are many other environmental and biological sample matrices. LC-MS/MS workflows are continuously developed to address these different matrices and the increasing number of required PFAS compounds and related analytes. There are methods and guides available, such as EPA 537.1, EPA 533, and DOD QSM 5.3, that can be used by laboratories directly or to assist with method development for the quantitation of PFAS compounds and replacement compounds (GenX). However, ultrashort-chain PFAS compounds (C2 and C3) are difficult for many methods, from a chromatographic perspective. The work presented here will focus on developing reversed phase chromatographic methodology for these ultrashort-chain PFAS compounds. Retention of these ultrashort-chain PFAS compounds is the main problem with typical HPLC methodology. Phenyl based HPLC stationary phases have significantly different selectivity than the typical aliphatic C18 or C8 HPLC columns. With optimized eluent components and the appropriate choice of pH, these columns can be used to retain ultrashort-chain PFAS compounds. Based on the EPA method flexibility rule, this methodology can be combined with many existing LC-MS/MS workflows