Evaluating the Robustness of the Zebron™ ZB-Dioxin GC Column for Dioxin Analysis in Challenging Matrices: Fish Oil and Fly Ash

New Organic Monitoring Techniques
Oral Presentation

Presented by K. Hope
Prepared by K. Hope1, M. MacLennan2, R. Dhandapani3, R. Jack3, R. Elabaji3
1 - Pacific Rim Laboratories, Inc., #103, 19575 55A Ave, Surrey, BC, V3S 8P8, Canada
2 - Pacific Rim Laboratories, 19575 55a Ave, SURREY, British Columbia, V3S 8P8, Canada
3 - Phenomenex, 411 Madrid Ave, Torrance, CA, 90501, United States


Contact Information: kjell@pacificrimlabs.com; 604-532-8711


ABSTRACT

Dioxin separation is challenging due to the presence of structurally similar analytes, co-elutions and other interfering compounds. Furthermore, a tough sample matrix often adds complexity to the analysis. There is a necessity to achieve high chromatographic resolution of dioxins and furans in order to meet separation criteria, irrespective of mass spectrometric approach. Presented in this study is an evaluation of robustness and selectivity of a gas chromatography (GC) column. Fish oil samples were spiked with Dioxin and Furan compounds and analyzed by GC-MS/MS to evaluate the column robustness across 100 injections. Environmental samples known to contain dioxins and furans (soil and fly ash) were analyzed by GC-HRMS to evaluate column robustness and selectivity across 30 injections. The results of the study showed a consistent enhanced chromatographic resolution of dioxins/furans co-elutions and minimal retention time shift of all analytes, demonstrating the robustness and selectivity of the GC stationary phase to challenging matrix components.