Wastewater Based Epidemiology for Monitoring Community Infection of SARS-CoV-2

SARS CoV-2 Wastewater Testing - State of the Science
Oral Presentation

Prepared by A. Pierri
Weck Laboratories, Inc., 14859 Clark Ave., Industry, CA, 91745, United States


Contact Information: agustin.pierri@wecklabs.com; 626-336-2139


ABSTRACT

Surveillance testing in communities is an important part of understanding community infection and transmission across a number of infectious diseases. Specifically, wastewater testing for SARS-CoV2 has emerged as an efficient, low-cost and reliable method for assessing current and trending infection levels as well as following trends to try to anticipate what hospital and ICU occupancies may look like.
One approach for this wastewater-based epidemiology (WBE) is the Quantitative Real Time Polymerase Chain Reaction assay. In this talk, we aim to discuss background and applicability of WBE with regards to the current pandemic, as well as one approach for wastewater testing, as it applies to SARS-CoV-2 detection. This talk will describe the sample concentration, extraction, and cleanup steps for isolating viral RNA from raw wastewater. We will also discuss the use of a multiplexed internal standard and process control which serves to improve the overall data quality and laboratory efficiency for WBE. Additionally, the implications of using WBE in other microbial detection and infectious disease monitoring will be addressed.