Application of a New WAX SPE Cartridge for The Analysis of PFAS in Drinking Water

Polyfluoroalkyl Substances (PFAS) in the Environment
Poster Presentation

Prepared by S. Shollenberger1, J. Simon2, O. Shimelis1, C. Aurand1
1 - MilliporeSigma, 585 N Harrison Rd., Bellefonte, PA, 16823, United States
2 - Merck KGaA, Frankfurter Str. 250, , Darmstadt, Germany

Contact Information:; 814-359-5476


Per- and polyfluoroalkyl substances (PFAS) are included in the monitoring list of drinking water contaminants across the globe due to their potential negative impact on human health. Solid phase extraction (SPE) using a weak anion exchange (WAX) cartridge followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) is the preferred method for the determination of select PFAS in drinking water in the low ppt (ng/mL) range.
In this study, Supelclean ENVI-WAX SPE cartridges were evaluated for use in the extraction of 25 short chain and emerging PFAS analytes from drinking water samples consistent with EPA Method 533. It was demonstrated that the WAX SPE cartridge have an extremely low PFAS background, which is essential to prevent contamination of the sample during the extraction procedure. Replicate extractions of each PFAS analyte using the WAX SPE cartridge were found to be well within the method recovery guidelines of 70-130%. Additionally, percent relative standard deviation (%RSD) values for replicate sample extractions were all found to be less than the 20% required by the method. This indicates that the WAX cartridge is an equivalent replacement for the required phase in EPA 533, in both chemical composition as well as required performance.