Challenges in Microbial Assays of Produced Waters

Oral Presentation

Prepared by K. McLeroy
M-I SWACO/Schlumberger, 3600 Briairpark Drive, Houston, Texas, 77042

Contact Information: lmcleroy@slb.com; 713-689-2084


ABSTRACT

Most often oil and gas produced waters are allowed to either accumulate in storage tanks or ponds until further disposed of or treated for reuse. During this detention time, bacterial growth can occur and proliferate. These bacteria in turn can cause negative effects on treatment and reuse applications. An inherent issue with culturing and monitoring for these bacteria is the complexity of the water chemistry matrices and the overall biomass of the system. These matrices can interfere with traditional bacteria growth media and colony color indicator broths. This presentation examines the various methodological approaches that are utilized in both field and laboratory analyses for bacteria in oil/gas produced waters and to facilitate discussions among industry leaders. Additional considerations will focus on what problems can occur with those methods and what future microbiological protocols will the industry need in the future.