Follow NEMC
 |
 |
 |
Improvement to Method 1623 to Detect Cryptosporidium and Giardia
Oral Presentation
Prepared by L. Villegas
CB & I, 26 W. Martin Luther King Dr., Cincinnati, OH, 45268
Contact Information: Villegas.Leah@epa.gov; 513-569-7886
ABSTRACT
One global public health challenge is detecting pathogens in water. Advances in laboratory procedures are allowing for better detection of pathogens. Like Cryptosporidium—which can cause gastrointestinal illness. The illness may be severe and sometimes fatal for people with weakened immune systems. The U.S. Environmental Protection Agency developed and validated Method 1623.1 as an effective procedure to improve Cryptosporidium recovery from challenging matrices using Filtration/IMS/FA. The substantial changes from the current Method 1623 are the addition of sodium hexametaphosphate, and a rinse to remove excess debris during immunomagnetic separation of the organisms. The Method 1623.1 inter-laboratory validation study consisted of 14 laboratories analyzing 4 reagent water and 4 source water samples. The Cryptosporidium mean recovery represented a ≥20 percentage point increase compared to the previous validation of Method 1623. The Giardia mean recoveries of approximately 40% were similar to the previous method validation. Laboratories performing Method 1623.1 are expected to see improved Cryptosporidium recoveries from challenging matrices and higher analytical confidence knowing they are meeting more stringent quality control.
Oral Presentation
Prepared by L. Villegas
CB & I, 26 W. Martin Luther King Dr., Cincinnati, OH, 45268
Contact Information: Villegas.Leah@epa.gov; 513-569-7886
ABSTRACT
One global public health challenge is detecting pathogens in water. Advances in laboratory procedures are allowing for better detection of pathogens. Like Cryptosporidium—which can cause gastrointestinal illness. The illness may be severe and sometimes fatal for people with weakened immune systems. The U.S. Environmental Protection Agency developed and validated Method 1623.1 as an effective procedure to improve Cryptosporidium recovery from challenging matrices using Filtration/IMS/FA. The substantial changes from the current Method 1623 are the addition of sodium hexametaphosphate, and a rinse to remove excess debris during immunomagnetic separation of the organisms. The Method 1623.1 inter-laboratory validation study consisted of 14 laboratories analyzing 4 reagent water and 4 source water samples. The Cryptosporidium mean recovery represented a ≥20 percentage point increase compared to the previous validation of Method 1623. The Giardia mean recoveries of approximately 40% were similar to the previous method validation. Laboratories performing Method 1623.1 are expected to see improved Cryptosporidium recoveries from challenging matrices and higher analytical confidence knowing they are meeting more stringent quality control.