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Multifaceted Approach to Arsenic Speciation Analysis of Plant and Animal Tissue
Oral Presentation
Prepared by R. Gerads, H. Gurleyuk
Applied Speciation and Consulting, LLC, 18804 Northcreek Parkway, Bothell, WA, 98011
Contact Information: russ@appliedspeciation.com; 425-483-3300
ABSTRACT
A multi faceted approach to the speciation of arsenic present in plant and animal tissues was applied for the quantitation of: arsenite, arsenate, monomethylarsonic acid, dimethylarsenic acid, trimethylarsine oxide, tetramethylarsonium ion, arsenocholine, arsenobetaine, thio-dimethylarsenite, roxarsone, arsenolipids, arsenosugars, p-arsinilic acid, and phenylarsonic acid. The extraction and analytical methods were specific to the target molecules and substrate. Comparison between various extraction methods was performed including, but not limited to: neutral pH, phosphate based extractions, enzymes, organic acids, polar solvent, and non-polar solvents. Certain extraction methods were proven to quantitatively decompose organic arsenic species to arsenate impacting the capacity to make educated decisions for ecological risk assessment. The findings from this research conclusively identified that the applied extraction and analytical methods must be specific to the substrate components and the target arsenic species. Analytical method used for quantitation of arsenic species included different chromatographic separation techniques (GC, IC, RP) coupled to an inductively coupled plasma collision reaction cell mass spectrometer.
Oral Presentation
Prepared by R. Gerads, H. Gurleyuk
Applied Speciation and Consulting, LLC, 18804 Northcreek Parkway, Bothell, WA, 98011
Contact Information: russ@appliedspeciation.com; 425-483-3300
ABSTRACT
A multi faceted approach to the speciation of arsenic present in plant and animal tissues was applied for the quantitation of: arsenite, arsenate, monomethylarsonic acid, dimethylarsenic acid, trimethylarsine oxide, tetramethylarsonium ion, arsenocholine, arsenobetaine, thio-dimethylarsenite, roxarsone, arsenolipids, arsenosugars, p-arsinilic acid, and phenylarsonic acid. The extraction and analytical methods were specific to the target molecules and substrate. Comparison between various extraction methods was performed including, but not limited to: neutral pH, phosphate based extractions, enzymes, organic acids, polar solvent, and non-polar solvents. Certain extraction methods were proven to quantitatively decompose organic arsenic species to arsenate impacting the capacity to make educated decisions for ecological risk assessment. The findings from this research conclusively identified that the applied extraction and analytical methods must be specific to the substrate components and the target arsenic species. Analytical method used for quantitation of arsenic species included different chromatographic separation techniques (GC, IC, RP) coupled to an inductively coupled plasma collision reaction cell mass spectrometer.