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Predicting Water Quality at Ohio Beaches Using qPCR
Oral Presentation
Prepared by M. Citriglia, N. Schafer, K. Greenwood
Northeast Ohio Regional Sewer District, 4747 East 49th Street, Cuyahoga Hts., Ohio, 44125
Contact Information: citrigliam@neorsd.org; 216-641-6000
ABSTRACT
The Northeast Ohio Regional Sewer District (NEORSD) has two GLRI grants that parallel each other. One grant focuses on Modeling at Urban Beaches the other grant focuses on testing various rapid methods. Villa Angela (VA) beach in Cleveland Ohio has made the National Resource Defense Council’s (NRDC) repeat offenders list. This list contains any beach that has had more than 25% of samples taken exceeding the Single Sample Maximum (SSM) of 235cfu/100ml from 2006 through 2011. Historical data generated by NEORSD indicates that VA beach exceeds the SSM on more than 40% of the samples taken. Additionally using the previous days E. coli results to determine water quality is only accurate 50% of the time. NEORSD has been using rapid method technology qPCR for the analysis of E. coli on samples collected at various beaches since 2007. NEORSD has compiled historical data from 2009 and 2011 and has determined that qCPR technology can be used to accurately predict the water quality at VA beach. A predictive model was created using the Virtual Beach Program created by the U.S. EPA. Samples are collected at 7:00am in the morning, delivered to the lab, processed and analyzed by qPCR. The E. coli results are determined from a standard curve and then entered into the model to predict the E. coli density for the day. The data is the entered on the Ohio Nowcast Site and used for public notification.
Oral Presentation
Prepared by M. Citriglia, N. Schafer, K. Greenwood
Northeast Ohio Regional Sewer District, 4747 East 49th Street, Cuyahoga Hts., Ohio, 44125
Contact Information: citrigliam@neorsd.org; 216-641-6000
ABSTRACT
The Northeast Ohio Regional Sewer District (NEORSD) has two GLRI grants that parallel each other. One grant focuses on Modeling at Urban Beaches the other grant focuses on testing various rapid methods. Villa Angela (VA) beach in Cleveland Ohio has made the National Resource Defense Council’s (NRDC) repeat offenders list. This list contains any beach that has had more than 25% of samples taken exceeding the Single Sample Maximum (SSM) of 235cfu/100ml from 2006 through 2011. Historical data generated by NEORSD indicates that VA beach exceeds the SSM on more than 40% of the samples taken. Additionally using the previous days E. coli results to determine water quality is only accurate 50% of the time. NEORSD has been using rapid method technology qPCR for the analysis of E. coli on samples collected at various beaches since 2007. NEORSD has compiled historical data from 2009 and 2011 and has determined that qCPR technology can be used to accurately predict the water quality at VA beach. A predictive model was created using the Virtual Beach Program created by the U.S. EPA. Samples are collected at 7:00am in the morning, delivered to the lab, processed and analyzed by qPCR. The E. coli results are determined from a standard curve and then entered into the model to predict the E. coli density for the day. The data is the entered on the Ohio Nowcast Site and used for public notification.